티타늄 상에 전기화학적 증착 방법을 이용한 소혈청알부민 함유 수산화인회석 형성 연구
A study on the formation of bovine serum albumin-containing hydroxyapatite on titanium using electrochemical deposition method
박규원, 최병한, 박영준, 송호준,
소속 상세정보
박규원 ( Park Kyu-Won ) - Chonnam National University School of Dentistry Department of Dental Materials
최병한 ( Choi Byung-Han ) - Chonnam National University School of Dentistry Department of Dental Materials
박영준 ( Park Yeong-Joon ) - Chonnam National University School of Dentistry Department of Dental Materials
송호준 ( Song Ho-Jun ) - Chonnam National University School of Dentistry Department of Dental Materials
Abstract
본 연구에서는 전기화학적증착(electrochemical deposition; ECD) 방법을 이용하여 티타늄 상에 유기물인 소혈청알부민 (bovine serum albumin; BSA)을 함유하는 수산회인회석(hydroxyapatite; HAp)을 코팅하였다. 본 연구를 위하여 티타늄 BSA를 첨가한 Ca와 P을 함유하는 37 ℃ 전해질에 티타늄 시편을 침척한 후 음전류(negative electric current)를 인가하였다. 전류의 세기를 ?10 mA, ?15 mA, ?20 mA로 변화시켜 E-BSA-10, E-BSA-15, E-BSA-20 시편을 제조하였다. 비교군으로서 ?15 mA의 전류를 인가하여 BSA를 함유하지 않은 E-15 시편을 제조하였다. 각 시편 표면에는 나노 sheet 형상의 결정들로 구성된 코팅막이 관찰되었으며, BSA를 함유한 시편들의 나노 결정의 크기가 E-15 시편에 비하여 더 컸다. 이 결정들은 인가전류 값이 클수록 크기는 작아지고 밀도는 더 높았다. 모든시편들은 HAp 결정구조를 가지고 있었다. BSA를 함유한 시편들은 c-축 방향으로 HAp 결정들이 우선 성장하는 경향을 보여주었고, 인가전류 값이 클수록 그 경향은 낮아졌다. FT-IR 스펙트럼에서 BSA에 기인한 amide peak들을 확인하였다. BSA를 함유한 시편들을 60시간 동안 인산완충생리식염수(phosphate-buffered saline; PBS)에 침적한 결과, c-축으로 정렬되었던 결정구조가 무질서해지는 것을 확인하였으며, 함유되어 있는 BSA는 HAp의 분해와 함께 천천히 용출되었다.
본 연구 방법은 향후 다양한 생물학적 인자들을 함유한 HAp를 티타늄 임플란트에 코팅하는 표면처리 방법을 개발하는데적용될 수 있을 것으로 기대된다.
In this study, the hydroxyapatite (HAps) containing bovine serum albumin (BSA) were coated on titanium using the electrochemical deposition (ECD) method. Negative electric current pulses were applied to titanium specimens at 37 ℃ using a Ca- and P-containing electrolyte in which BSA was added E-BSA-10, E-BSA-15, and E-BSA-20 groups were prepared by changing the electric current to ?10 mA, ?15 mA, and ?20 mA, respectively. As a comparative group, an E-15 specimen was prepared by applying a current of -15 mA to the specimen in Ca- and P-containing electrolyte without BSA. A coating film composed of nanosheet-shaped crystals was observed on the surface of each specimen, and the size of the nanocrystals on the BSA-containing groups was larger than that of the E-15 group. These crystals were smaller in size and higher in density as the applied current values were increased. All specimens had HAp crystalline structure. BSA-containing specimens showed a tendency of preferential growth of HAp crystals in c-axis orientation, and this tendency decreased as the applied current values were increased. In the FT-IR spectrum, amide peaks that corresponds to BSA were identified. As a result of soaking the BSA-containing group specimens in phosphate-buffered saline (PBS) for 60 hours, it was observed that the crystal structure aligned with the c-axis was disordered, and BSA was released slowly with the decomposition of HAp. This study is expected to be applied to the development of a surface treatment method for coating titanium implants with HAp containing various biological factors in the future.
키워드
티타늄; 표면개질; 전기화학적증착; 수산화인회석; 소혈청알부민
Titanium; Surface modification; Electrochemical deposition; Hydroxyapatite; Bovine serum albumin
원문 및 링크아웃 정보
등재저널 정보